Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 01/24/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Caught on branches of trees located on the Georgia Southern University – Armstrong Campus. Collected around 530PM using the beat tray and stick. Weather was roughly between 50-55 degrees Fahrenheit at time of collection. |
| Putative identification | Arthropoda Hexapoda Insecta Psocodea |
Methods |
|
| Extraction kit | none |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Order was not previously categorized locally and not many specimens worldwide have been documented leading my predictions to be negative. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Psocodea was found to be negative for Wolbachia. |
Water Boatman