ZEACESP23 – Psocoptera

April 16, 2023

Sample information
Picture
Photos by: Evie H.
Location
Collection date 01/24/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Caught on branches of trees located on the Georgia Southern University – Armstrong Campus. Collected around 530PM using the beat tray and stick. Weather was roughly between 50-55 degrees Fahrenheit at time of collection.
Putative identification Arthropoda Hexapoda Insecta Psocodea

Methods
Extraction kit none
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Order was not previously categorized locally and not many specimens worldwide have been documented leading my predictions to be negative.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Psocodea was found to be negative for Wolbachia.
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