Pill Bug

October 14, 2020

Sample information
Picture
Photos by: Emilia S.
Location
Collection date 09/24/2020
Captive / Cultivated? Wild-caught
Group Hillsboro High School
Observations

I found this pill bug/rolly polly under a wet flower pot. It was collected in the mid-afternoon around 4:20 pm. It was in the higher 60s lower 70s outside. There were lots of rolly pollies in this area, as well as under wet leaves and old wood.
Putative identification Arthropoda Crustacea Malacostraca Isopoda Armadillidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I am highly confident that this insect does not have Wolbachia because when reading and looking at the results from the gel electrophoresis under different contrast levels, there was no band present. That means that there was no Wolbachia present in the insect to create a band.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Armadillidae was found to be negative for Wolbachia.
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