|Captive / Cultivated?||Wild-caught|
|Group||Hillsboro High School|
I found this pill bug/rolly polly under a wet flower pot. It was collected in the mid-afternoon around 4:20 pm. It was in the higher 60s lower 70s outside. There were lots of rolly pollies in this area, as well as under wet leaves and old wood.
|Putative identification||Crustacea Malacostraca Isopoda Armadillidae|
|Extraction kit||DNeasy (Qiagen)|
|DNA extraction location||Whole arthropod|
|Single or Duplex PCR|
|Gel electrophoresis system||MiniPCR|
|Explanation of confidence level||
I am highly confident that this insect does not have Wolbachia because when reading and looking at the results from the gel electrophoresis under different contrast levels, there was no band present. That means that there was no Wolbachia present in the insect to create a band.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Armadillidae was found to be negative for Wolbachia.|