ThriveScholars-Spider-DATeam

August 9, 2023

Sample information
Picture
Location
Collection date 08/08/2023
Captive / Cultivated? Wild-caught
Group Thrive Scholars
Observations

Very small spider
Putative identification Arthropoda Hexapoda Insecta

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain GelRed
Gel images
Protocol notes

We followed protocol on website.

  • The spider sample is in lane 2 of both the top and bottom rows of the gel.
  • The top row used arthropod primers while the bottom row used Wolbachia primers. In addition, the 8th well of the top and bottom rows is the DNA ladder because the 1st well of both rows didn’t have enough DNA ladder sample.

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

It is medium because Wolbachia DNA was detected in the spider sample, however, when using arthropod primers, there was no evidence from the electrophoresis that the spider was an arthropod when we know, in fact, that it is.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Insecta was found to be postive for Wolbachia.
Report Inappropriate Post