Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/08/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Thrive Scholars |
| Observations | |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Formicidae Lasius Lasius niger |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TBE |
| DNA stain | GelRed |
| Gel images |
|
| Protocol notes | Followed the Wolbachia website protocol. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Low |
| Explanation of confidence level | The gel and the bands were streaky; there might have been problems with the DNA concentrations being too high. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Lasius niger was found to be postive for Wolbachia. |
Water Boatman