Thrive Summer 2023 – Black Ant; Group: Where’s Kevin

August 9, 2023

Sample information
Picture
Photos by: Shital O.
Location
Collection date 08/08/2023
Captive / Cultivated? Wild-caught
Group Thrive Scholars
Observations
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae Lasius Lasius niger

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain GelRed
Gel images
Protocol notes

Followed the Wolbachia website protocol.

Results
Wolbachia presence Yes
Confidence level Low
Explanation of confidence level

The gel and the bands were streaky; there might have been problems with the DNA concentrations being too high.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Lasius niger was found to be postive for Wolbachia.
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