Grasshopper

September 19, 2023

Sample information
Picture
Photos by: brayan N
Location
Collection date 11/02/2023
Captive / Cultivated? Wild-caught
Group Antioch High School
Observations

I caught it around the school i was walking out and i was looking around for some arthropod and i saw him jumping so i caught him
Putative identification Arthropoda Hexapoda Insecta Orthoptera

Methods
Extraction kit Wizard SV (Promega)
DNA extraction location Rear half
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

when i was smashed the bug in the tube it was turn green.  when i start to spin it it would be skin and the DNA was thick.

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

i think mine does have some what Wolbachia because i did some research and it said half of the population of grasshopper has Wolbachia. i said my confidence level is low because we used the same tip when getting the dna.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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