Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/02/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Antioch High School |
| Observations | I caught it around the school i was walking out and i was looking around for some arthropod and i saw him jumping so i caught him |
| Putative identification | Arthropoda Hexapoda Insecta Orthoptera |
Methods |
|
| Extraction kit | Wizard SV (Promega) |
| DNA extraction location | Rear half |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TBE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | when i was smashed the bug in the tube it was turn green. when i start to spin it it would be skin and the DNA was thick. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | i think mine does have some what Wolbachia because i did some research and it said half of the population of grasshopper has Wolbachia. i said my confidence level is low because we used the same tip when getting the dna. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Water Boatman