Asian Lady beetle (Harmonia axyridis)

Sample information
Picture	Photos by: Lucinda M
Location
Collection date	09/13/2023
Captive / Cultivated?	Wild-caught
Group	Edmund Burke School
Observations	orange, elytra with approx 12-15 black spots, 6 legs, small antennae, round and small
Putative identification	Arthropoda Hexapoda Insecta Coleoptera Coccinellidae Harmonia Harmonia axyridis
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	MiniOne
Buffer	TBE
DNA stain	GelGreen
Gel images
Protocol notes	DNA Extraction Arthropod was hard to crush despite the size, no errors were made Gel electrophoresis Arthropod CO1: no errors made Gel Electrophoresis Wolbachia: no errors made just slight pipetting misstep Lane 1 (DNA ladder), lane 2 (B1), lane 3 (B2), lane 4 (B3), lane five (B4) lane 6 (WOL+), lane 7 (WOL -), lane 8 (water) The controls were successful
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	The procedure was followed very closely and there was net even a faint line present, the gel was completely Wolbachia-negative.
Wolbachia 16S sequence
Arthropod COI sequence	Download FASTA Download AB1
Summary	The Harmonia axyridis was found to be negative for Wolbachia.

The Wolbachia Project Database