Asian Lady beetle (Harmonia axyridis)

November 26, 2023

Sample information
Picture
Photos by: Lucinda M
Location
Collection date 09/13/2023
Captive / Cultivated? Wild-caught
Group Edmund Burke School
Observations

orange, elytra with approx 12-15 black spots, 6 legs, small antennae, round and small
Putative identification Arthropoda Hexapoda Insecta Coleoptera Coccinellidae Harmonia Harmonia axyridis

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

DNA Extraction Arthropod was hard to crush despite the size, no errors were made

Gel electrophoresis Arthropod CO1: no errors made

Gel Electrophoresis Wolbachia: no errors made just slight pipetting misstep

Lane 1 (DNA ladder), lane 2 (B1), lane 3 (B2), lane 4 (B3), lane five (B4) lane 6 (WOL+), lane 7 (WOL -), lane 8 (water)

The controls were successful

 

 

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The procedure was followed very closely and there was net even a faint line present, the gel was completely Wolbachia-negative.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
Summary The Harmonia axyridis was found to be negative for Wolbachia.
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