Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/13/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Edmund Burke School |
| Observations | orange, elytra with approx 12-15 black spots, 6 legs, small antennae, round and small |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera Coccinellidae Harmonia Harmonia axyridis |
Methods |
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| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | DNA Extraction Arthropod was hard to crush despite the size, no errors were made Gel electrophoresis Arthropod CO1: no errors made Gel Electrophoresis Wolbachia: no errors made just slight pipetting misstep Lane 1 (DNA ladder), lane 2 (B1), lane 3 (B2), lane 4 (B3), lane five (B4) lane 6 (WOL+), lane 7 (WOL -), lane 8 (water) The controls were successful
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Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | The procedure was followed very closely and there was net even a faint line present, the gel was completely Wolbachia-negative. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
|
| Summary | The Harmonia axyridis was found to be negative for Wolbachia. |
Water Boatman