LOGACFF23-CD Odonata

November 7, 2023

Sample information
Picture
Photos by: Cole D.
Location No map location data available.
Collection date 08/23/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Caught with an aerial net near bushes on a hot and sunny day.
Putative identification Arthropoda Insecta Odonata

Methods
Extraction kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

My controls were correct yet I still only had a single band. This makes me confident my insect did not have wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Odonata was found to be negative for Wolbachia.
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