LOGACFF23-AJR

November 7, 2023

Sample information
Picture
Location
Collection date 08/22/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

The insect was caught on the wall beside the Science Center at Georgia Southern University Armstrong Campus on 8/22/23 at 1:35 PM.

 

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Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

A DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

There was no question that my insect was negative for Wolbachia, as it comes from a historically low Wolbachia infected order, and the gel electrophoresis results controls performed as expected. No band was present for the Wolbachia gene, demonstrating that it was negative.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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