LOGACFF23AGR Hymenoptera

November 8, 2023

Sample information
Picture
Photos by: Amanda R
Location
Collection date 08/22/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Collected 8/22/23 at 1:30pm on Georgia Southern University Armstrong campus

Environment: Ungnadia speciosa (Mexican buckeye tree)

Method: stick and sheet
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Positive, negative, and NTC controls worked as expected. Absence of Wolbachia DNA in Hymenoptera sample is consistent with previously collected local prevalence data.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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