Sample information |
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Picture |
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Location | |
Collection date | 08/01/2023 |
Captive / Cultivated? | Wild-caught |
Group | Stevens Point Area Senior High |
Observations | Caught near a strand of mature red pine, white pine, oak, and cherry trees. The season was late summer. It was 70°. |
Putative identification | Arthropoda Hexapoda Insecta Diptera Culicidae Culex |
Methods |
|
Extraction kit | Edwards Buffer |
DNA extraction location | Whole arthropod |
Single or Duplex PCR | Single Reaction |
Gel electrophoresis system | Standard electrophoresis system |
Buffer | TBE |
DNA stain | GelRed |
Gel images |
|
Protocol notes | |
Results |
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Wolbachia presence | No |
Confidence level | High |
Explanation of confidence level | There was a band for the positive control and not one for my mosquito. |
Wolbachia 16S sequence | |
Arthropod COI sequence |
|
Summary | The Culex was found to be negative for Wolbachia. |