Pug Moth

April 2, 2024

Sample information
Picture
Photos by: Maya B
Location
Collection date 03/25/2024
Captive / Cultivated? Wild-caught
Group Scientists in Residence
Observations

The moth was on the wall of my front porch in the early morning.
Putative identification Arthropoda Insecta Lepidoptera Geometridae Eupithecia

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I had a bright band for wolbachia.
Wolbachia 16S sequence Download FASTA    Download AB1
Arthropod COI sequence Download FASTA    Download AB1
Summary The Eupithecia was found to be postive for Wolbachia.
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