Carpenter Ant

March 30, 2024

Sample information
Picture
Photos by: William H.
Location
Collection date
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

This is a very small ant. It was found travelling across a brick sidewalk outside. It is black. These ants live underground.
Putative identification

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The controls worked. I ran two tests, one to test for the insect’s DNA as a positive control and the other to test for wolbachia. I got a positive result for the insect DNA, so I know that I did the procedure correctly.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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