DEMAFsp24-22 Dermaptera

April 11, 2024

Sample information
Picture
Photos by: Ava H
Location
Collection date 01/24/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Completed on 1/24/24 at 1-1:30 pm, class completed in-lab insect collection.

Location: field by the greenhouse on the GSU Armstrong campus

Environment/ Habitat: Terrestrial (land-based)/ damp due to rain

Location: Field, looking under logs and tall grass with sweep net (collection technique)

Discovery: Many bugs (ants, beetles, worms, and many unknown species)

Temperature: moderately warm, more bugs were out and were easier to catch
Putative identification Arthropoda Hexapoda Insecta Dermaptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

My confidence level is high. After finishing my project viewers can see that the data controls worked. Not only that, they also lined up with my prediction/local data which states that Wolbachia will not be precent in Dermaptera.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Dermaptera was found to be negative for Wolbachia.
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