Spiders(2) – Trainor, Small Spider Used

January 31, 2025

Sample information
Picture
Photos by: Molly T.
Location
Collection date 10/15/2024
Captive / Cultivated? Wild-caught
Group John Bapst Memorial High School
Observations
Putative identification Arthropoda Chelicerata Arachnida Araneae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain Other
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

There was no results for the positive control Arthropod DNA or for the spider Arthropod DNA (both of which for sure should have been positive) which leads me to believe that some part of the PCR process went wrong. Maybe when I was preparing my samples I didn’t get enough positive control DNA and spider DNA. Either way, the fact that those samples didn’t come through makes my confidence level low.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Araneae was found to be negative for Wolbachia.
Report Inappropriate Post