Sample information
Picture	Photos by: Kacidy M., Isabella N.
Location
Collection date	01/02/2025
Captive / Cultivated?	Wild-caught
Group	Penn State Lehigh Valley
Observations	In the landscape
Putative identification	Arthropoda Diplopoda
Methods
Extraction kit	DNeasy (Qiagen) blood + tissue kit with modification P2 for ATL + N3 for AL
DNA extraction location	Partial abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system Bio-Rad
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Diplopoda was found to be negative for Wolbachia.