Wolbachia-positive Chrysoperla species

May 5, 2025

Sample information
Picture
Photos by: Zachary L.
Location
Collection date 04/01/2025
Captive / Cultivated? Wild-caught
Group College of Eastern Idaho
Observations

Arthropod was collected alive in an interior suburban environment.
Putative identification Arthropoda Insecta Neuroptera Chrysopidae Chrysoperla

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Controls used in the Gel Electrophoresis process (rows 1 & 2 WspecF, rows 5 & 6 CO1) appeared clearly and without clear issue, as well as the sample EP.
Wolbachia 16S sequence Download FASTA    Download AB1
TGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTGCCATCAGGTAATGCTGAGTACTTTAAGGAAACTGCCAGTGATAAGCTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCTTTATGGAGTGGGCTACACACGTGCTACAATGGTGTCTACAATGGGTTGCAAGGTGCGCAAGCCTAAGCTAATCCCTAAAAGACATCTCAGTTCGGATTGTACTCTGCAACTCGAGTACATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
ATTTGATCTGGATTAGTAGGTACAAGATTAAGTTTATTGATTCGAGCTGAATTAGGTCAACCAGGTTCTTTAATTGGTGATGATCAAATTTATAATGTAATTGTTACAGCTCATGCTTTTATTATAATTTTCTTTATAGTAATACCTATTGTAATTGGAGGTTTTGGTAATTGATTAGTTCCTTTAATACTAGCTGCTCCTGATATAGCTTTTCCACGAATAAATAATATAAGTTTCTGAATATTACCTCCTTCATTAACTTTATTACTTGCTTCTTCTATAGTAGAAAGAGGAGCTGGGACTGGTTGAACAGTTTACCCTCCTTTATCATCAAGAATTGCACATGCTGGAGCTTCTGTGGATTTAGCTATTTTTAGTTTACACCTTGCCGGAATCTCATCAATTTTAGGTGCAGTAAATTTCATTACAACAGTAATTAATATACGATTAAGTTATATAACATTAGACCGTATACCATTATTTGTATGATCAGTAGTAATTACAGCTTTATTATTATTACTTTCATTACCTGTATTAGCGGGTGCTATTACTATGTTATTAACTGATCGTAATTTAAATACTTCATTTTTTGATCCTGCAGGAGGAGGAGATCCAATTTTATATCAACATTTATTTTGATTTTTTGGTCACCC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Chrysoperla was found to be postive for Wolbachia.
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