Condylostylus GRP2

May 22, 2025

Sample information
Picture
Photos by: Sabina Q.
Location
Collection date 05/12/2025
Captive / Cultivated? Wild-caught
Group California Academy of Mathematics and Science
Observations
  • The arthropod was caught in a garden.
  • It was green and had long legs.
Putative identification Arthropoda Insecta Diptera Dolichopodidae Condylostylus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system Bio-Rad
Buffer 1X TAE
DNA stain 100X Fast Blast
Gel images
Protocol notes

The arthropods were caught and then ground to proceed to the DNA extraction and purification process. Once our experimental and control samples were prepped, we moved on to the PCR protocol. Samples and our PCR cocktail were added to each of our PCR tubes, which were then put into our MiniOne PCR machine. Finally, loading dye was added to the separated portions of our PCR samples and was loaded into our gel. Our final gel was stained with 100X Fast Blast and put onto a light box for imaging.

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

The results of this arthropod are shown in lane 2 of the gel. The sample resulted in a band for the CO1 arthropod gene, but none for the 16S rRNA gene for Wolbachia. As there wasn’t a 16S rRNA gene in lane 4 for the positive DNA control that ensures that the PCR was successful, there isn’t sufficient evidence to make a conclusion about the presence of Wolbachia in the arthropod.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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