Gymnoclytia Occidua, Tachinid Fly

September 11, 2025

Sample information
Picture
Location
Collection date 09/11/2025
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

6 legs

around 1 cm

brown

line streaks on the back
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain Cyber Stain
Gel images
Protocol notes

Lane 1: SGSG03W1 –>grasshopper

Lane 2: SGSG03W2 –> stinkbug

Lane 3:  SGSG03W3 –> positive control

Lane 4: SGSG03W5 –> negative control

Lane 5: SGSG03W6 –> DNA control

Lane 6: SGSG03W6 –> water

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

My sample tested negative for Wolbachia as the bacteria’s DNA was not detected. This could indicate that my stinkbug simply did not Wolbachia living inside it. Comparing it to the PCR and the control group, it could be seen there was no presense of Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
TNGANNTNNNNCAGGAATAGTGGGANCTNCNNTAAGTATTATAATTCGAACTGAATTAGGACACCCCG GATCATNATTGGAAATGATCAAATTTATAATGTTGTTGTTACTGCTCATGCCTTCATTATAATTTTTTTTATAGTAATAC CAATTATAATTGGAGGATTTGGTAATTGATTAGTGCCATTAATATTTGGAGCCCCTGATATAGCTTTCCCTCGAATAAAT AATATAAGATTTTGATTATTACCCCCTGCATTAACTCTCCTNATAATAAGTAGAATAATTGAAACCGGAGCTGGAACCGG TTGAACTGTATATCCCCCTTTATCTTCTAATATTGCCCATGGAGGAGCTTCTGTAGATTTGGCTATTTTTTCTTTACATT TAGCAGGAGTTTCTTCAATTTTAGGAGCNGTTAATTTTATTACAACANTTATTAATATACGANCNNNAGGAATTANNTTN GANNNNNNACCNTTATTTGTTNGNNNTGTTGAANTTACNNCTTNNNNTTNNTNNTTATCNTTACCANNACTANNNGGANN TATAACAANNTNATNACCNAACNNAANTTTTANAACTTCTTTTTTGGNCCCCNNAGGAGNNGNAAATCNATTTTGATNTC NNCATTANTCTGANTTTTTNNNCN
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Arthropoda was found to be negative for Wolbachia.
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