Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/11/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Penn State Lehigh Valley |
| Observations | Found underneath brush, sedentary when captured |
| Putative identification | Arthropoda Insecta Coleoptera Carabidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | 1X TAE |
| DNA stain | Cyber Stain |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | Fairly confident, reviewed results with professor |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
TTNNNNGNAGNNTGATNNGGATAGTAGGAACTTCTTTAAGTCTATTAATTCGAGCAGAATTAGGGAATCCAGG ATCACTAATTGGTGATGATCAAATTTATAATGTTATTGTTACTGCCCATGCTTTTATTATAATTTTTTTTATAGTAATAC CTATTATAATTGGTGGATTTGGAAATTGATTAGTTCCTTTAATATTAGGAGCACCTGATATGGCCTTTCCTCGAATAAAT AATATAAGTTTTTGACTTTTACCTCCTTCTTTAACACTTCTTCTTATAAGAAGACTAGTAGAAAGAGGAGCAGGTACAGG TTGAACTGTATACCCCCCTTTATCTTCAGGAATTGCCCATAGAGGTGCTTCAGTAGATTTAGCTATTTTTAGTCTTCACT TAGCGGGGGTATCCTCTATTTTAGGAGCAGTTAATTTTATTACTACTATTATCAATATACGATCAGTAGGTATAACATTT GAACGAATACCACTATTTGTTTGATCAGTAGGTATTACAGCTTTACTATTACTTTTATCATTACCAGTTTTAGCAGGAGC TATTACTATGTTATTAACAGATCGAAATTTAAATACTTCCTTTTTTGATCCTGCAGGAGGGGGAGACCCAATTCTTTACC AACATTTATTTTGATTTTTNG
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Carabidae was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid