Roly Poly

May 3, 2021

Sample information
Picture
Photos by: Eric W.
Location
Collection date 03/30/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

It rolls into a ball.

It has antennae.

It has 14 legs.

It had a see through back.
Putative identification Arthropoda Crustacea Malacostraca Isopoda

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

The pellet was dark and visible.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

This is my first time extracting DNA.

Arthropod control—A didn’t seem to work.

The positive and negative controls in the Wolbacchia didn’t seem to work.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Isopoda was found to be negative for Wolbachia.
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