Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/31/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | The ant was a giant compared to others, it was brown with black strips on its back, the weather was sunny and it was caught in a forest, this ant was found in a log |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | The only mistake was that the gel for Wolbachia was made wrong so the answers are not completely clear the pellet was white and covered the entire bottom of the tube some people also poked the gel while inserting into pcr machine |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | Arthropod controls-not sure about the bands in Wolbachia DNA and negative arthropod Wolbachia controls-not sure about arthropod negative band |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hymenoptera was found to be postive for Wolbachia. |
Extraction and Analysis of Wolbachia from Halyomorpha halys
Tick
Roly-Poly
Idaho Beetle
Grammonota