Carpenter Ant

Sample information

Picture
Location
Collection date 03/31/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

The ant was a giant compared to others, it was brown with black strips on its back, the weather was sunny and it was caught in a forest, this ant was found in a log

Putative identification Hexapoda Insecta Hymenoptera

Methods

Extraction kit Edwards Buffer
DNA extraction location Abdomen
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

The only mistake was that the gel for Wolbachia was made wrong so the answers are not completely clear the pellet was white and covered the entire bottom of the tube some people also poked the gel while inserting into pcr machine

Results

Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

Arthropod controls-not sure about the bands in Wolbachia DNA and negative arthropod

Wolbachia controls-not sure about arthropod negative band

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be postive for Wolbachia.
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