Roly-Poly

May 4, 2021

Sample information
Picture
Photos by: Ellie H.
Location
Collection date 04/01/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

They were about a half an inch long each . They weren’t very active when I caught them under a moist log. They are both gray in color and were close together when found.
Putative identification Arthropoda Crustacea Malacostraca Isopoda

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

When doing the gel part the gel crumbled causing us to let the gel sit for a little longer than ten minutes. The pellet was gray and large in size.

 

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Arthropod controls, not sure about the bands on walbachia dna, and negative arthropod, Walbachia controls, not sure about arthropod negative band.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Isopoda was found to be negative for Wolbachia.
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