Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/31/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | Hybrid Russian honey bee, found outside hive, collected at about 5 pm, partly cloudy, 53 degrees |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Apidae Apis Apis mellifera |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Added more Edwards’s buffer because there was to little supernatant Pellet: large, yellowish brown gells were not properly prepared, the comb was inserted before gel was added, this led to inconsistently shaped wells. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | Not sure if there was enough dna to process |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Apis mellifera was found to be negative for Wolbachia. |
Water Boatman