Armadillidium vulgare

May 8, 2026

Sample information
Picture
Entry by: Nithin S.
Location
Collection date 11/07/2025
Captive / Cultivated? Wild-caught
Group Walton High School
Observations

Rolls up after any danger type taxis. Moves at a slow pace. Body is gray with white stripes.
Putative identification Arthropoda Malacostraca Isopoda Armadillidae Armadillidium Armadillidium vulgare

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer 1X TAE
DNA stain GelGreen
Gel images
Protocol notes

Crushed the full organism, as it was about the size of a rice grain, and cutting the abdomen would have been very difficult. We then centrifuged and only extracted DNA. Took the extracted DNA and ran it through Gel Electrophoresis. Results yielded that there was no Wolbachia in this organism.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I am able to confidently say that this organism is Wolbachia negative due to there being no banding in the Wolbachia gel meaning that there is not wolbachia DNA in that organism.
Wolbachia 16S sequence Download FASTA   
Arthropod COI sequence Download FASTA    Download AB1
TTGAGCAGGGGCTGTTGGAACTGCCCTTAGAATAATTATTCGCACTGAATTAGGACAACCTGGAAGTTTAATTGGAGATGACCAGATTTACAATGTAATTGTAACTGCTCATGCTTTTGTAATAATTTTTTTTATAGTAATACCTATTATAATTGGTGGATTTGGTAATTGGTTAATTCCATTAATACTAGGAGCCCCAGATATGGCTTTTCCACGAATAAATAATATAAGATTTTGACTTTTACCCCCTTCTTTAACTTTATTGCTTAGAAGTGGGTTGGTTGAAAGAGGAGTAGGAACAGGATGGACAGTATATCCTCCGCTGGCGTCTAGAATTGCTCATAGAGGAGCATCTGTAGATTTAGGTATTTTTTCTCTCCACCTTGCTGGAGCTTCTTCTATTTTAGGGGCTGTGAATTTTATTACTACTATAATTAATATACGAGCAGCTGGAATTAGAATAGACCGTGTTCCTTTATTTGTTTGATCAGTATTAGTAACGGCTGTACTTTTACTTTTATCCCTACCTGTATTAGCAGGAGCTATTACTATATTATTAACTGATCGAAATTTAAATACCTCTTTTTTTGACCCTAGGGGAGGAGGAGACCCTATCCTTTATCAACACTTATTTTGATTTTTTGGTCACCCTGGAAGTTTAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Armadillidium vulgare was found to be negative for Wolbachia.
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