Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/07/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Walton High School |
| Observations | Black, smooth shell, smaller body, segmented into three, six legs, small clippers or fangs. |
| Putative identification | Arthropoda Insecta Coleoptera Carabidae Laemostenus Laemostenus complanatus |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | 1X TAE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | The quality score for my CO1 sequence was 52 marking 634 bases which is high confidence. Anything above a 40 quality score warrants a high confidence level. Initially the controls on our gels provided the results that we expected with the bands present. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download AB1
|
| Summary | The Laemostenus complanatus was found to be negative for Wolbachia. |
Centipede – MJAR
Fruitfly – MJAR
Ant – MJAR
Mosquito – MJAR
Bumblebee – MJAR