Roly-Poly

May 5, 2021

Sample information
Picture
Location
Collection date 04/02/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

It looked like a normal roly-poly. It was still alive when I collected it. It had a hard shell and 14 legs; and it was a dark brown color.
Putative identification Arthropoda Crustacea Malacostraca Isopoda

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

The pellet was very small and hard to see. It looked clear though.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

DNA ladder in arthropod gel was not loaded properly. Positive and negative controls did not work. Wolbachia gel control did not work. Maybe problems with DNA extraction.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Isopoda was found to be negative for Wolbachia.
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