Roly Poly

May 5, 2021

Sample information
Picture
Photos by: Cooper R
Location
Collection date 03/29/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations
  • Has 3 sections
  • tail is black
  • can’t see legs
  • most of the body is tanish brown
Putative identification Arthropoda Crustacea Malacostraca Isopoda Armadillidae Armadillidium Armadillidium vulgare

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Pellet was very clear

May not have pipetted right

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

DNA ladder in arthropod gel was not loaded properly

positive and negative controls did not work

Wolbachia gel control did not work maybe problems with DNA extraction
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Armadillidium vulgare was found to be negative for Wolbachia.
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