Sample information

Collection date 04/01/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy

The queen ant is black and brown. It has a small structure. Has 1 pair of wings. The wings are only brown. The bug has 6 legs that are brown and black. Four wings, sparse ribs; front wings are larger than back wings. Chewing type for the mouth part. Completed Metamorphosis.

Putative identification Arthropoda Hexapoda Insecta Hymenoptera


Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Overall I think I’ve made no mistakes, however I struggled on the DNA extraction process. The pellet was very small and it was visible. The pellet was a brown color


Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

There may have been a switch between the Wolbachia DNA and the negative arthropod control in the Wolbachia band. In the Wolbachia band the negative arthropod control should not have a band.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be postive for Wolbachia.
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