|Captive / Cultivated?||Wild-caught|
|Group||Hampton Roads Academy|
The queen ant is black and brown. It has a small structure. Has 1 pair of wings. The wings are only brown. The bug has 6 legs that are brown and black. Four wings, sparse ribs; front wings are larger than back wings. Chewing type for the mouth part. Completed Metamorphosis.
|Putative identification||Hexapoda Insecta Hymenoptera|
|Extraction kit||Edwards Buffer|
|DNA extraction location||Whole arthropod|
|Single or Duplex PCR|
|Gel electrophoresis system||MiniOne|
Overall I think I’ve made no mistakes, however I struggled on the DNA extraction process. The pellet was very small and it was visible. The pellet was a brown color
|Explanation of confidence level||
There may have been a switch between the Wolbachia DNA and the negative arthropod control in the Wolbachia band. In the Wolbachia band the negative arthropod control should not have a band.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Hymenoptera was found to be postive for Wolbachia.|