BRI-ANH Order Diptera

April 16, 2026

Sample information
Picture
Location
Collection date 01/29/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Fly was caught with a sweep net in bushes near Georgia Southern University parking lot.
Putative identification Arthropoda Insecta Diptera

Methods
Extraction kit
DNA extraction location Abdomen/cut:partial
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

My confidence level is low since during our gel electrophoresis, my insect band didn’t show, so I’m unable to determine anything about my insect with certainty.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Diptera was found to be negative for Wolbachia.
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