Formica pallidefulva

April 20, 2026

Sample information
Picture
Entry by: Cole H.
Location
Collection date 09/02/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Collected by Fall 2025 students.
Putative identification Arthropoda Insecta Hymenoptera Formicidae Formica Formica pallidefulva

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I am confident in it after the gel was ran professionally the second time. At first, I did not notice any Wolbachia due to it being not too clear. The second time it was ran, Wolbachia came back and I had found out what it was. I also had a 100% ID match once it was ran.
Wolbachia 16S sequence
GAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
GATATAGCTTATCCTCGTATAAATAATATAAGATTTTGACTTTTACCTCCTTCCATCACTCTATTACTTT TAAGAAACTTTATTAATGATGGTACTGGAACTGGATGAACTATCTATCCTCCTTTAGCTTCAAATATTTT TCATAATGGACCTTCTGTAGACTTAACAATTTTTTCTCTTCATATTGCCGGTATATCATCTATTTTAGGA GCTATTAATTTTATTTCAACAATTCTAT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Formica pallidefulva was found to be postive for Wolbachia.
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