Lasius niger

May 21, 2026

Sample information
Picture
Entry by: Yara L. & Magdalena V.
Location
Collection date 03/26/2026
Captive / Cultivated? Wild-caught
Group Gymnasium Immensee
Observations

Caught in snowy conditions on a paved sidewalk.
Putative identification Arthropoda Insecta Hymenoptera Formicidae Lasius Lasius niger

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer 1X TAE
DNA stain SYBR Green
Gel images
Protocol notes

1st from left is DNA of Lasius niger
2nd form left is another insect
3rd from left is a positive arthropod control
4th from left is negative arthorpod control
5th from left is gDNA
6th from left is water
7th from left is a DNA-marker

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

All the negative and positive controls worked except for the 4th one on the left.

For the first and second ones were used 400 microlitre of TE-buffer instead of only 50 microlitre for the DNA-Elution. It had no influence on the results, because they were still clearly visible.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Lasius niger was found to be negative for Wolbachia.
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