GRP 10 Cluster Fly (Pollenia rudis)

May 22, 2026

Sample information
Picture
Entry by: Evan T
Location
Collection date 04/13/2026
Captive / Cultivated? Wild-caught
Group California Academy of Mathematics and Science
Observations

Caught on the outside of a classroom wall.
Putative identification Arthropoda Insecta Diptera Polleniidae Pollenia

Methods
Extraction kit QIAGEN DNeasy kit
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system 1X Agarose Gel Electrophoresis
Buffer 1X TAE
DNA stain 100X Fast Blast
Gel images
Protocol notes

1% agarose gel

ran at 165 volts, 75 mAmps, for 65 min

Results
Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

Well #3, containing the Cluster Fly sample, visualized no results, meaning an error in PCR or DNA extraction, likely being the latter.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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