Sample information
Picture	Entry by: Muhammed Emin T
Location
Collection date	04/21/2026
Captive / Cultivated?	Wild-caught
Group	Winterset High School
Observations	eastern black carpenter ant
Putative identification	Arthropoda Insecta Hymenoptera Formicidae Camponotus Camponotus pennsylvanicus
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	MiniPCR
Buffer	1X TBE
DNA stain	Cyber Green
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	i followed up all steps on the protocols and no band was present
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Camponotus pennsylvanicus was found to be negative for Wolbachia.