Sample information

Arthropod was found in the outdoors – an area dense with grass in May. The ground was clear with an abundance of plants, an area familiar with many arthropods of the Polistes denomination. Weather was not harsh, and the specimen had a smooth brown exterior with black markings throughout.,

Methods

DNA was extracted using the DNeasy (Qiagen) Blood and Tissue Kit. PCR was performed as a single reaction for both CO1 and Wolbachia specific primers. Gel electrophoresis was run using the MiniPCR gel electrophoresis system with TBE buffer and SYBR Safe DNA stain. The CO1 gel was used confirm successful arthropod DNA amplification, and the WSpec gel was used to test for the presence of Wolbachia. We also chose the DNA ladder to be in lade 10 instead of lane 6.

Results

I said the the Paper Wasp was Wolbachia negative due to no clear band present. However, due to the band not even being faintly visible, I am highly confident that the Paper Wasp was Wolbachia negative.