Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 05/29/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Pingry School |
| Observations | Caught in a sugar and soap trap next to a tree at the front of the school woods. Suburban area. |
| Putative identification | Arthropoda Insecta Diptera Polleniidae Pollenia Pollenia rudis |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | agarose gel electrophoresis |
| Buffer | 1X TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | Gel electrophoresis results showed positive for Wolbachia. Matched 373/373 with Wolbachia endosymbiont on NCBI BLAST after gene sequencing. |
| Wolbachia 16S sequence | Download FASTA
|
| Arthropod COI sequence | Download FASTA
|
| Summary | The Pollenia rudis was found to be postive for Wolbachia. |
Centipede – MJAR
Fruitfly – MJAR
Ant – MJAR
Mosquito – MJAR
Bumblebee – MJAR