Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 06/29/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Pingry School |
| Observations | Found on the side of a small wooden bridge over a stream on a walking trail in the woods at school. Suburban area. |
| Putative identification | Arthropoda Arachnida Araneae Dictynidae Nopalityna Nopalityna sublata |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | agarose gel electrophoresis |
| Buffer | 1X TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Gel electrophoresis results showed negative for Wolbachia. Did not match with any Wolbachia sequences on NCBI BLAST after gene sequencing. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
|
| Summary | The Nopalityna sublata was found to be negative for Wolbachia. |
Centipede – MJAR
Fruitfly – MJAR
Ant – MJAR
Mosquito – MJAR
Bumblebee – MJAR