September 10, 2026

Sample information
Picture
Entry by: Jazlene L.
Location No map location data available.
Collection date 09/10/2026
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

The insect is red legged, has antennas.
Putative identification Arthropoda Hexanauplia

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes
  1. DNA Ladder
  2. Arthropod 1
  3. Arthropod 2
  4. Positive arthropod control
  5. Negative arthropod control
  6. DNA Control
  7. water

Both bands were present; however, Wolbachia was not present.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The controls to ensure accuracy did not show, therefore, these results are correct.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hexanauplia was found to be negative for Wolbachia.
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