Lepidoptera

September 10, 2026

Sample information
Picture
Entry by: SR
Location
Approximate location: 40.56, -75.40
Collection date 09/10/2026
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

The moth is small, tan color, brown lines on the wings, brown on the tips of the wings, found in bus, east to catch when in net, relaxed, the Inaturalist app had 100 confidence on what insect it was.
Putative identification Arthropoda Insecta Lepidoptera

Methods
Extraction kit Qiagen DNeasy Blood + Tissue Kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes
  1. DNA Standard
  2. O2 GO HN + skB1 + O2GOHN + SRB1 – Arthropod 1
  3. O2 GO HN + skB2 + O2GOHN + SRB2 – Arthropod 2
  4. O2 GO HN + skB3 + O2 GOHN + SRB3 – (+) Control
  5. O2 GO HN + skB4 + O2 GOHN + SRB4 – (-) Control
  6. O2 GO HN + sk + O2 GOHN + sk – (DNA Control)
  7. O2 GO HN + sk + O2GOHN + sk – water
  8. Empty

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Nothing was amplified in our Electrophoresis Gel’s so no bands were produced.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Lepidoptera was found to be negative for Wolbachia.
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