Lemidoptera (Shield Bug)

September 10, 2026

Sample information
Picture
Entry by: Katelynn F.
Location No map location data available.
Collection date 09/10/2026
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

Light green stink bug the size of a dime found in a patch of long grass
Putative identification Arthropoda Insecta Lepidoptera

Methods
Extraction kit Qiagen DNeasy Blood and Tissue Kit
DNA extraction location Reproduction
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes
Group 2D
Lanes 1-8

Lane 1- DNA Standard

Lane 2- Arthropod #1 Wol + COI1

Lane 3- Arthropod #2 Wol + COI1

Lane 4- Wol Pos Wol + COI1

Lane 5- Wol Neg Wol + COI1

Lane 6- gDNA control Wol + COI1

Lane 7- water control Wol + COI1

Lane 8- Empty
Group 2E
Lane 9- DNA Standard

Lane 10- Arthropod #1 Wol + COI1

Lane 11- Arthropod #2 Wol + COI1

Lane 12- Wol Pos Wol + COI1

Lane 13- Wol Neg Wol + COI1

Lane 14- gDNA control Wol + COI1

Lane 15- water control Wol + COI1

Lane 16 – Empty

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Lepidoptera was found to be negative for Wolbachia.
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