Bumblebee

September 10, 2026

Sample information
Picture
Entry by: Melanie V
Location No map location data available.
Collection date 09/10/2026
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

Black-yellow fuzz. Six legs, two wings and its body.

Lithobius Forficatus- (centipede)
Putative identification Arthropoda Insecta Hymenoptera Apidae Bombus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location
Single or Duplex PCR Single Reaction
Gel electrophoresis system standard electro
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes

lane 1- no clear band

lane 2- faint band

lane 3- two bright bands

lane 4- faint bands

lane 5- two bright bands

lane 6- faint band

lane 7- faint band

lane 8- DNA ladder

 

lane 1- no clear band

lane 2- no clear band

lane 3- two bright bands

lane 4- band present

lane 5- two bright bands

lane 6- no clear band

lane 7- band present

lane 8- DNA ladder

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA   
TTNATCNNNNNTAATTGGAACTGGCCTTAGCCTTCTTATTCGGCTAGAATTAAGACAGCCTGGAACATTAATTGGAGATG ATCAAATTTATAATGTGATTGTTACTGCCCACGCCTTCATTATAATTTTTTTTATAGTTATACCAGTTATAATTGGAGGT TTTGGAAATTGACTTGTCCCTTTAAAATTAGGGGCCCCAGACATANCCNTTCCTCGAATAAATAATATANNANTTTGGNT CCTTCCCCCCTCCCTAACTCTCTTGCTATGTTCAGCAGCAGTAGAAAAAGGGGCCGGAACGGGATGAACCGTTTATCCCC CCTTATCCTCTAATATTTCCCATAGAGGAGCATCCGTTGATATAACAATTTTTTCATTACACCTCGCCGGTGCATCATCA ATTTTAGGGGCAATTAACTTTATTTCCCCAATTATTAATATGCAAACTANAGGAATATCATTTGAACGANTTCCNNNANT NGTATGATCCGTAAAAATTACAGTTATTCTACTCCTCCTTTCCCTCCCAGTATTAGCCGGAGCAATTACTATACTATTAA CTGACCGAAACTTANNTACTATATTTTTTCGACCCTACANGANGAGGAGACCCAATTTTATACCAACACTTATTTTTGAT TTTTTGNTCACC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Bombus was found to be negative for Wolbachia.
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