Subfamily Pentatominae

September 10, 2026

Sample information
Picture
Entry by: Nahla Y.
Location No map location data available.
Collection date 09/10/2026
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

Dark shell, shield shape shell, underside tan with spotted legs which are segmented into three sections. It was caught within the bushes, the sweep net was swished back and forth within the bushes and caught, it was crawling within the bush.
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system agarose gel electrophoresis
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Lane 1 is for this specific Arthropod

  1. DNA Standard
  2. Wol Arthropod 1 DANY1
  3. Wol Arthropod 2 DANY2
  4. Positive control
  5. Negative control
  6. DNA control
  7. Water control
  8. empty
  9. DNA Standard
  10. Wol Arthropod 1 SNMV1
  11. Wol Arthropod 1 SNMV2
  12. positive control
  13. negative control
  14. DNA control
  15. Water control
  16. empty

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Band sequence
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
GNANTTGATCNGGACTAGTAGGAATAATAATAAGAATAATTATTCGAATAGAACTATCTCAACCAGGATCAATAATCAAA AATGACCAAATTTATAACACAATTGTAACTTCACATGCATTTGTAATAATCTTTTTTATAGTTATACCAATTATAATTGG TGGTTTTGGAAACTGGCTTGTTCCTCTTATAATTGGAGCACCAGATATAGCTTTCCCACGAATAAACAATATAAGTTTTT GACTTTTACCAATATCAATTACATTATTAATTGCAAGATCAACAGCAGCTTCAGGAACAGGAACAGGATGAACTGTATAC CCACCTCTTTCGGCTCAAGAAGCACATTCAGGACCATCAGTAGACTTAACAATTTTTTCATTACACATTGCAGGTATTAG ATCAATTATAGGAGCAATTAATTTTATTTCAACCATTATAAATATACGAATCAAAGGAATAACAATAGAAAAAACCCCCT TATTTTGTTGATCAGTACTAATTACAGCAATNCCTTTTACTAGTTTCTTTANCAGTACTANCCGGAGCANCNNNANATTN NNNNNNANNGAAANNNANTANNNNTTTTNNATCNTCNGNNNGNGG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Arthropoda was found to be negative for Wolbachia.
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