JWO 05 – Locust Borer

September 11, 2026

Sample information
Picture
Entry by: Jake O
Location
Approximate location: 40.29, -76.89
Collection date 09/03/2026
Captive / Cultivated? Wild-caught
Group Commonwealth Charter Academy
Observations

On plant in woods area of HACC Parking Lot.

Red legs, long antennae, yellow and black stripes
Putative identification Arthropoda Insecta Coleoptera Cerambycidae Megacyllene Megacyllene robiniae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer 1X TBE
DNA stain GelGreen
Gel images
Protocol notes

Followed duplex PCR & Minione gel procedures. Refer to label 5 on gel image.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Bright band seen in Wolbachia specific gene.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Megacyllene robiniae was found to be postive for Wolbachia.
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