DEM-IMM

November 2, 2026

Sample information
Picture
Location
Approximate location: 31.97, -81.16
Collection date 08/31/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

The specimen was identified as a lovebug in the order Diptera. It had a small black head with antennae, a dark thorax with one pair of wings and six legs, and a long segmented black abdomen. The insect was collected using a sweep net.

 
Putative identification Arthropoda Insecta Diptera

Methods
Extraction kit tweezers
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA was extracted from the abdomen of the specimen using reagents provided in the lab.

 

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I am confident in my results because the controls worked as expected and the gel results were clear. DNA was successfully extracted from the lovebug specimen, but no Wolbachia band was detected. Based on these results, I found no evidence of Wolbachia infection in my specimen.

 
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Diptera was found to be negative for Wolbachia.
Report Inappropriate Post