Sample information |
|
| Picture |
|
|---|---|
| Location | Approximate location: 31.97, -81.16 |
| Collection date | 09/01/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | one pair of wings |
| Putative identification | Arthropoda Insecta Diptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | I have high confidence in the negative result becuase no band was deteted at the expected wolbachia 16s . The presence of the CO1 band would confrim that the sample contained amplifiable DNA, supporting a true negative rather than a failed reaction |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Diptera was found to be negative for Wolbachia. |
DEM-NJR Hymenoptera
(no title)
DEMALJ blattodea
Diptera
MATSKB Diptera