Sample information

The roly-polys were together under a log, where it was cold and wet. The roly-polys liked to stay away from sunlight, mostly likely in dry and dark places in the dirt. I observed that it had 7 sets of legs, a hard plated dark grey exoskeleton, an oval body, and curled into a ball when touched, so I was able to identify that it was a pillbug.

Methods

I think that the DNA was not thoroughly cleaned and that enough was pulled out of the cells in the reproductive tissues of my arthropod.

Results

Through gel electrophoresis, I saw that my pill bug tested inconclusive for Wolbachia because the RFLPS for my arthropod DNA did not show when we interpreted the gel under a fluorescent light. Because of the temperature and the way the gel was loaded with the small amount of DNA from my pillbug, I was unable to see if my arthropod was negative or positive for Wolbachia.