Orthoptera

April 26, 2022

Sample information
Picture
Location
Collection date 04/10/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

6 legs, 2 head antennas, exoskeleton, 3 butt antennas
Spots and strips with a deep brown and beige color
Putative identification Arthropoda Insecta Orthoptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

The positive arthropod control did not show an arthropod band
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Orthoptera was found to be postive for Wolbachia.
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