Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/10/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | The arthropod was hiding under a rock from the sun, searching for a cooler environment. It rolled up into a ball for protection when it was disturbed for the observation. It had seven pairs of legs. It had simple eyes, uropods, a pair of prominent antennae. It had an exoskeleton with palates. |
| Putative identification | Arthropoda Crustacea Malacostraca Isopoda |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | The PCR was working correctly. The concentration of the gel might have been too high. This might have lead to errors in resolving the different bands. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | The positive arthropod control did not show an arthropod band. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Isopoda was found to be negative for Wolbachia. |
Water Boatman