|Captive / Cultivated?||Wild-caught|
|Group||Hampton Roads Academy|
The arthropod was hiding under a rock from the sun, searching for a cooler environment. It rolled up into a ball for protection when it was disturbed for the observation. It had seven pairs of legs. It had simple eyes, uropods, a pair of prominent antennae. It had an exoskeleton with palates.
|Putative identification||Arthropoda Crustacea Malacostraca Isopoda|
|Extraction kit||Edwards Buffer|
|DNA extraction location||Abdomen|
|Single or Duplex PCR||Single Reaction|
|Gel electrophoresis system||MiniOne|
The PCR was working correctly. The concentration of the gel might have been too high. This might have lead to errors in resolving the different bands.
|Explanation of confidence level||
The positive arthropod control did not show an arthropod band.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Isopoda was found to be negative for Wolbachia.|