Armadillidium vulgare

April 26, 2022

Sample information
Picture
Location
Collection date 04/10/2022
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

The arthropod was hiding under a rock from the sun, searching for a cooler environment. It rolled up into a ball for protection when it was disturbed for the observation. It had seven pairs of legs. It had simple eyes, uropods, a pair of prominent antennae. It had an exoskeleton with palates.
Putative identification Arthropoda Crustacea Malacostraca Isopoda

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

The PCR was working correctly. The concentration of the gel might have been too high. This might have lead to errors in resolving the different bands.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

The positive arthropod control did not show an arthropod band.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Isopoda was found to be negative for Wolbachia.
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