Sample information
Picture
Location
Collection date	06/10/2022
Captive / Cultivated?	Wild-caught
Group	Hampton Roads Academy
Observations	Ladybug was red
Putative identification	Arthropoda Hexapoda Insecta Coleoptera
Methods
Extraction kit	Edwards Buffer
DNA extraction location	Abdomen
Single or Duplex PCR
Gel electrophoresis system	MiniOne
Buffer	TBE
DNA stain	GelGreen
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Coleoptera was found to be negative for Wolbachia.