Pill bug

April 27, 2022

Sample information
Picture
Photos by: Marcy L.
Location
Collection date 04/05/2022
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

6 Legs, metallic shell.
Putative identification Arthropoda Crustacea Malacostraca Isopoda

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

The DNA pellet was tiny.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I had an arthropod band, but the postitive control did not show an arthropod band.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Isopoda was found to be negative for Wolbachia.
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