Scolops sulcipes- Partridge Bug

December 1, 2022

Sample information
Picture
Photos by: Chase Hartzell, Heather Ahlstrom
Location
Collection date 09/06/2022
Captive / Cultivated? Wild-caught
Group College of Eastern Idaho
Observations

Scolops sulcipes- Partridge Bug- Crawling in dirt in the shade by the path

Description: Curious, Dirt Brown with patterned markings, very camouflaged

Body length: 0.9cm Found by Carly Hartzell

UTM Coordinates: 12T 0492041 4797270

Arthropod: ?  Wolbachia: +
Putative identification Arthropoda Hexapoda Insecta Hemiptera Dictyopharidae Scolops Scolops sulcipes

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

DNA Annotated protocol in Wolbachia project Lab2: DNA Extraction Edward’s Buffer

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

We tested and retested as well as put a sample together for the Wolbachia Project Lab to identify and we received a sequence that was a match.
Wolbachia 16S sequence
>Wol13 CGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTGCTATCAGGTAATGCTGAGTACTTTAAGGAAACTGCCAGTGATAAGCTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCTTTATGGAGTGGGCTACACACGTGCTACAATGGTGTCTACAATGGGCTGCAAGGTGCGCAAGCCTAAGCTAATCCCTAAAAGACATCTCAGTTCGGATTGTACTCTGCAACTCGAGTACATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
>CO1_13_CspecF GTTTATGATCAGGATTACTAGGAACAATAAGAAGAATAATTATTCGTATAGAACTTTTACAACCAGGATCAATAATTAAAAATGACCAAATTTATAATACCATTGTTACATCACATGCCTTCATTATAATTTTCTTTATAGTTATACCAATTATAATTGGAGGATTCGGAAATTGATTAGTTCCAATAATAATTGGTGCACCAGATATAGCATTTCCACGAATAAATAACATAAGATTTTGATTATTACCCCCCTCAATAACATTACTAATCATAAGATCAATTACAGGATCAGGAACAGGAACAGGATGAACAATTTATCCCCCCTTATCTAACCAAATAGCACATTCAGGGCCATCAGTTGACTTAACTATTTTTTCATTACATATTGCAGGAATTAGATCAATTCTAGGAGCAATTAACTTTATCTCAACTATTACAAATATACGACCAAAACCTATAACAATAGAAAATACACCACTTTTCTGTTGATCAGTTCTAATTACAGCAATTTTATTATTAGTATCATTACCAATTCTAGCAGGAGCTATTACTATACTATTAACGGATCGAAACTTTAATACATCATTTTTTGACCCATCAGGAGGAGGGGACCCAATCTTATATCAACACCTATTTTG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Scolops sulcipes was found to be postive for Wolbachia.
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